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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in many tumors, which results in chromosome instability, and therefore may contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a significant job in endorsing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants had been reported but specific nucleotide sequence data just isn't still offered.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide typically consists of the epitope identified with the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is certain.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which add into the temporal coordination of each mitotic party. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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